產品簡介

anti-His Tag Mouse Monoclonal antibody（鼠單克隆抗體）在Western Blotting中使用內參其實就是在WB過程中的另外用內參對應的抗體檢測內參，這樣在檢測目的產物的同時可以檢測內參的表達，由于內參在各組織和細胞中的表達相對恒定，借助檢測每個樣品內參的量就可以用于校正上樣誤差，這樣半定量的結果才更為可信。

英文名稱      anti-His Tag Mouse Monoclonal antibody

規  格           100ul

稀釋比例       WB 1:3000 IP 1:200 IF 1:1000

濃  度           1mg/ml

保  存           Store at -20°C. Avoid freeze / thaw cycles.Stable for one year from the date of shipment.

儲存液        PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 61% Glycerol

性  狀           Lyophilized or Liquid

克隆類型      Monoclonal Antibody

宿  主           Mouse

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

說明書

Anti-His Tag Mouse Monoclonal Antibody (3C5)

Catalog No. M1001020

Size 100μl

Storage: Store at -20°C. Do not aliquot the antibody.

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His)

residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags

can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers

identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

Molecular Weight: N/A

Application: WB 1:1500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user.

Specificity: The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag

fusion proteins.

Form: Liquid ,1mg/ml

Storage buffer: PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol

Relative Products

M1001010 Anti- HA Tag Mouse Monoclonal Antibody(2C7)

M1001020 Anti-His Tag Mouse Monoclonal Antibody(3C5)

M1001030 Anti-GFP Tag Mouse Monoclonal Antibody(2H7)

M1001040 Anti-GST Tag Mouse Monoclonal Antibody(2C7)

M1001050 Anti- cMyc Tag Mouse Monoclonal Antibody(2C5)

M1001060 Anti- Flag Tag Mouse Monoclonal Antibody(1B3)

RG001010 Anti- HA Tag Rabbit Polyclonal Antibody

RG001020 Anti- His Tag Rabbit Polyclonal Antibody

RG001030 Anti- GFP Tag Rabbit Polyclonal Antibody

RG001040 Anti- GST Tag Rabbit Polyclonal Antibody

RG001050 Anti- cMyc Tag Rabbit Polyclonal Antibody

RG001060 Anti- Flag Tag Rabbit Polyclonal Antibody

Solarbio

Beijing Solarbio Science & Technology Co., Ltd

/82

Http://www.solarbio。。ｃｏｍ