產品簡介

Human IFN-gamma索萊寶ELISA試劑盒的優勢：
1，包被的酶標板單板可拆（能拆分成12個8孔的酶標條）
2，提供免費代測代檢服務，代出實驗數據
3，發文章高獎勵
4，磁鐵可吸附式包裝盒，包裝精美耐用

Human IFN-gamma 

Human IFN-gamma

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is captured by the coated antibody after incubation. Following extensive washing, a biotin-conjugate antibody specific for IFN-γ is added to detect the captured IFN-γ protein in sample. For signal development, horseradish peroxidase (HRP)-conjugated Streptavidin is added, followed by Tetramethyl-benzidine (TMB) reagent. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm